Passengers on the Seymour and Shepparton lines are advised of longer journey times during March and early-April, as works progress on the Shepparton Line Upgrade.
Coaches replace trains between Southern Cross, Seymour and Shepparton from Wednesday March 8 to Saturday April 1.
Passengers are urged to check the PTV app or website before travelling and to allow up to 60 minutes extra for their journey.
Coaches will then replace trains between Seymour and Shepparton only until the last service on Thursday April 6.
From Sunday April 2 to Thursday April 6, coaches will depart Shepparton up to 35 minutes earlier than usual train times to ensure connection with trains at Seymour.
Replacement coaches will be a combination of express, semi-express and stopping all stations services, and a temporary timetable will be available from the V/Line website to help passengers plan ahead.
Albury Line trains will operate as normal, except over a three day period from Saturday March 18 to Monday March 20 when the Australian Rail Track Corporation completes planned maintenance at various locations between Melbourne and Sydney.
During this time, crews will carry out signalling work, safety testing and driver training, putting the finishing touches on stage two of the Shepparton Line Upgrade.
V/Line will undertake works including the installation of new equipment for the new signalling system between Craigieburn and Seymour, and its annual maintenance along the line.
The Shepparton Line Upgrade is delivering faster, more frequent and reliable services and has enabled the introduction of modern VLocity trains.
Real-time traffic conditions are available at traffic.vicroads.vic.gov.au or via the VicTraffic mobile app. The latest public transport information and network status is available at ptv.vic.gov.au or in the PTV mobile app.