Police are investigating the circumstances surrounding a fatal collision in Craigieburn on Thursday night.
Emergency services were called to reports two females had been struck by a car on the Hume Freeway just after 7.30pm.
It is believed the pair had stopped to assist an injured animal before they were struck.
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A 30-year-old Beveridge woman died at the scene.
A 30-year-old Thomastown woman was airlifted to hospital but died a short time later.
The driver of the vehicle remained at the scene and assisted police.
The exact circumstances of the collision are being investigated.