Police are investigating a fatal crash near Tallarook that occurred earlier this month.
It is believed a truck came off the Hume Freeway, near Sharpes and Taylors Road, and became wedged under a tree at about 4am on January 4.
Emergency services attended the scene but the driver could not be revived.
Anyone who witnessed the collision, has dashcam footage or information is urged to contact Crime Stoppers 1800 333 000 or submit a confidential report online www.crimestoppersvic.com.au.